The pichia pastoris recombinant strain was induced by methanol addition to the culture medium Sds - page分析表明,在63kd處有明顯的蛋白質(zhì)特征帶。
Expression of cyt1aa from bacillus thuringiensis in bacillus thuringiensis var . tenebrionis strain and the insecticidal activity of the recombinant strain 基因在擬步甲亞種菌株中的表達(dá)及重組菌株殺蟲活性研究
The gene recombinant strain no . 42 ca n ' t generate ampramycin , which indicated that the cloned gene is involved in apramycin biosynthesis in s . tenebrarius 通過接合轉(zhuǎn)移的方法將質(zhì)粒pzxb014導(dǎo)入黑暗鏈霉菌h6中,篩選基因發(fā)生重組的菌株。
Finally , the growth conditions , growth curves , fermentation conditions of recombinant strains and the biological properties of purified recombinant protein were investigated 最后對重組菌的生長條件、生長曲線、發(fā)酵條件和表達(dá)產(chǎn)物的體外生物學(xué)活性進(jìn)行了研究。
Some important factors of high density fermentation of genetic engineering microorganisms including the construct of recombinant strains , culture conditions , growth inhibitor and the process control were described 摘要闡述了基因工程菌高密度發(fā)酵工藝的幾個主要影響因素,包括重組菌構(gòu)建、培養(yǎng)條件、生長抑制因數(shù)以及它們的控制技術(shù)。